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For two plasmid vectors to exist in one cell, they need to have compatible replication origins. In addition, they should have different antibiotic resistance markers to be selected within a cell. Our vectors belong to two compatibility groups A and B. The vectors are not compatible with each other within a compatibility groups. Vectors from different compatibility groups are compatible with one another. They may be used to co-express two or more proteins in one cell.

Compatibility groups

A (pACYC origin)

B (pBR322 or pUC origins)

pCA21a, pCA32a, pTA21a

pAB21a, pAU21a, pAU32a, pAU32b

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