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Special Media
 

High density bacterial E.coli culture media for plasmid and protein production
DNAGroTM, ProGroTM, AutoXTM, DetoXTM, InduXTM, and SecProTM media

High density bacterial E.coli culture media

bullet DNAGroTM medium: Support E.coli culture to OD600 = 30 - 50. Increase plasmid DNA yield 10 times over LB
bullet ProGroTM medium: Support E.coli culture to OD600 = 30 - 50. Increase protein yield 10 times over LB
bullet AutoXTM medium: Support E.coli culture to OD600 = 30 - 50. Increase protein yield 10 times over LB without adding IPTG
bullet DetoXTM medium: Reduce protein toxicity and support E.coli culture to OD600 = 30
bullet InduXTM medium: Support E.coli culture to OD600 = 30. Increase protein yield, solubility, stability and activity
bullet SecProTM medium: Increase secretary protein expression over 100 times than LB or TB

Features and benefits of high density culture media

bulletGrows E.coli cell density to OD600 = 30 to 50 in a shake flask (compared to OD600=2 to 3 in LB) which is comparable to that may be obtained from a regular fed-batch fermentation
bulletIncreases protein or plasmid DNA yield 10 times or higher
bulletIncrease secretory protein expression 100 times or higher
bulletpH is maintained close to optimal (pH 7) for E.coli culture up to OD 600 = 30
bulletContains trace metals, minerals, and vitamins, some of which are needed for E.coli culture and others which of may serve as prosthetic groups, co-factors or ligands for expressed recombinant proteins. These special media increase yield, solubility, stability, and activity of recombinant proteins

Applications of high density culture media

bulletHigh yield plasmid production, 10 times over LB
bulletHigh yield phage production, 10 times over LB
bulletHigh yield protein production, 10 times over LB
bulletHigh yield secretory expression, 100 times over LB or TB
bulletToxic protein expression
bulletIncrease protein solubility
bulletIncrease protein stability
bulletIncrease protein activity

Medium Name

Applications
DNAGroTM culture medium Increases plasmid and phage DNA yields 10 times over LB
ProGroTM culture  medium Increases protein yield, solubility, stability and activity
AutoXTM culture medium Increases protein yield, solubility, stability and activity; automatic protein expression  without adding IPTG
DetoXTM culture medium Reduces protein toxicity and support E.coli culture to OD600 = 30
InduXTM culture medium Increases protein yield, solubility, stability and activity
SecProTM culture medium Increases secretary protein expression over 100 times than LB or TB

More information for high density culture media...>
More information about yield, solubility, stability, and activity...>

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High density bacterial E.coli culture medium DNAGroTM
For plasmid DNA and phage production

For any given plasmid (copy number of the plasmid is determined), there are two ways to increase plasmid yield. The first way is to increase the cell density or the number of cells. The other way is to increase the percentage of cells containing the plasmid. Adding chloramphenicol to the medium may amplify some plasmids but not others. E.coli culture medium DNAGroTM is proprietarily formulated for plasmid DNA and phage production. Most commonly-used E.coli strains can reach OD600 = 30 in a shake flask container in this  medium. Recombinant protein cannot be expressed in this medium because the medium composition inhibits protein induction by IPTG, arabinose, or any sugar-based inductions. Protein induction may be achieved in this medium if heat or salt inducible promoters are used. Ten times or higher cell density may be obtained from this medium when compared to that from LB broth when culture conditions are closely followed. Plasmid or phage DNA yields will increase accordingly if there is sufficient selection.

High density culture medium DNAGroTM

Catalog # Price (US$) Add to Cart   
DNAGroTM liquid bacterial culture Medium
500 ml/bottle, 1 bottle
40101 40
DNAGroTM liquid bacterial culture Medium
500 ml/bottle, 12 bottles
40102 384
DNAGroTM liquid bacterial culture Medium
1 liter/bottle, 1 bottle
40103 78
DNAGroTM liquid bacterial culture Medium
1 liter/bottle, 12 bottles
40104 748
DNAGroTM powdered bacterial culture Medium
powder for 1 liter/pouch, 1 pouch
40105 70
DNAGroTM powdered bacterial culture Medium
powder for 10 liters, 2 liters/pouch, 5 pouches
40106 560

Click on above product names for literatures of the high density bacterial E.coli culture medium DNAGroTM

MSDS of the high density bacterial E.coli culture medium DNAGroTM

Liquid culture medium MSDS
Powder culture medium MSDS

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High density bacterial E.coli culture medium ProGroTM
For recombinant protein production

E.coli culture medium ProGroTM is proprietarily formulated for recombinant protein production. Most commonly used E.coli strains can reach OD600 = 30 in a shake flask container in this  medium. Recombinant proteins can be expressed in this medium regardless whether they are induced by IPTG, sugar, heat or salt. Ten times or higher cell density may be obtained in this medium than that from LB broth when culture conditions are closely followed. Recombinant protein yield will increase accordingly if there is sufficient selection. This culture medium contains trace metals, mineral, and vitamins that may be needed for solubility, stability, and activity of a recombinant protein.

High density culture medium DNAGroTM

Catalog # Price (US$) Add to Cart   
ProGroTM liquid bacterial culture Medium
500 ml/bottle, 1 bottle
40111 40
ProGroTM liquid bacterial culture Medium
500 ml/bottle, 12 bottles
40112 384
ProGroTM liquid bacterial culture Medium
1 liter/bottle, 1 bottle
40113 78
ProGroTM liquid bacterial culture Medium
1 liter/bottle, 12bottles
40114 748
ProGroTM powdered bacterial culture Medium
powder for 1 liter/pouch, 1 pouch
40115 70
ProGroTM powdered bacterial culture Medium
powder for 10 liters, 2 liters/pouch, 5 pouches
40116 560

Click on above product names for literatures of the high density bacterial E.coli culture medium ProGroTM

MSDS of the high density bacterial E.coli culture medium ProGroTM

Liquid culture medium MSDS
Powder culture medium MSDS

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High density bacterial E.coli culture medium AutoXTM
For automatic recombinant protein expression without adding IPTG

E.coli culture medium AutoXTM is proprietarily formulated for recombinant protein production. Most commonly used E.coli strains can reach OD600 = 30 in a shake flask container in this  medium. Recombinant proteins can be automatically expressed in this medium if they are induced by IPTG. Induction occurs after cell density reaches OD600 = 10. A specific inducer must be added if the recombinant protein is not induced by IPTG or lactose. Cell density ten times or higher may be obtained in this medium when compared to that from LB broth when culture conditions are closely followed. Recombinant protein yield will increased accordingly if there is sufficient selection. This culture medium contains trace metals, mineral, and vitamins that may be needed for solubility, stability, and activity of a recombinant protein.

High density culture medium DNAGroTM

Catalog # Price (US$) Add to Cart   
AutoXTM liquid bacterial culture Medium
500 ml/bottle, 1 bottle
40121 40
AutoXTM liquid bacterial culture Medium
500 ml/bottle, 12 bottles
40122 384
AutoXTM liquid bacterial culture Medium
1 liter/bottle, 1 bottle
40123 78
AutoXTM liquid bacterial culture Medium
1 liter/bottle, 12 bottles
40124 748
AutoXTM powdered bacterial culture Medium
powder for 1 liter/pouch, 1 pouch
40125 70
AutoXTM powdered bacterial culture Medium
powder for 10 liters, 2 liters/pouch, 5 pouches
40126 560

Click on above product names for literatures of the high density bacterial E.coli culture medium AutoXTM

MSDS of the high density bacterial E.coli culture medium AutoXTM

Liquid culture medium MSDS
Powder culture medium MSDS

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High density bacterial E.coli culture medium DetoXTM
For toxic recombinant protein production

DetoXTM E.coli culture medium is proprietarily formulated for toxic protein production. Most commonly used E.coli strains can reach OD600 = 30 in a shake flask container in this  medium. This medium is designed for E.coli cell culture and propagation, but not for recombinant protein expression or production. The inhibitors in this medium will prohibit protein expression induced by IPTG, lactose, arabinose, or any other sugars. After cells reach inducible OD (5 to 20 for most cell strains or recombinant proteins), other high density culture media such as InduXTM or ProGroTM should be transferred to the cell pellet and induction may be performed in these media instead. Using common bacterial culture medium such as LB will result in lower yield. The medium has no effect on protein expression induced by heat or salt. This culture medium contains trace metals, mineral, and vitamins that may be needed for solubility, stability, and activity of a recombinant protein.

High density culture medium DNAGroTM

Catalog # Price (US$) Add to Cart   
DetoXTM liquid bacterial culture Medium
500 ml/bottle, 1 bottle
40131 40
DetoXTM liquid bacterial culture Medium
500 ml/bottle, 12 bottles
40132 384
DetoXTM liquid bacterial culture Medium
1 liter/bottle, 1 bottle
40133 78
DetoXTM liquid bacterial culture Medium
1 liter/bottle, 12 bottles
40134 748
DetoXTM powdered bacterial culture Medium
powder for 1 liter/pouch, 1 pouch
40135 70
DetoXTM powdered bacterial culture Medium
powder for 10 liters, 2 liters/pouch, 5 pouches
40136 560

Click on above product names for literatures of the high density bacterial E.coli culture medium DetoXTM

MSDS of the high density bacterial E.coli culture medium DetoXTM

Liquid culture medium MSDS
Powder culture medium MSDS

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High density bacterial E.coli culture medium InduXTM
For toxic recombinant protein production with DetoXTM medium

InduXTM E.coli culture medium is proprietarily formulated for recombinant protein production. Most commonly used E.coli strains can reach OD600 = 30 in a shake flask container in this medium. Recombinant proteins can be expressed in this medium regardless whether they are induced by IPTG, any other sugars, heat or salt. Ten times or higher cell density may be obtained in this medium than that from LB broth when culture conditions are closely followed. Recombinant protein yield will increase accordingly if there is sufficient selection. No IPTG is needed if the recombinant protein is induced by IPTG or lactose. A specific inducer must be added at induction if the protein is not induced by IPTG or lactose. This culture medium contains trace metals, mineral, and vitamins that may be needed for solubility, stability, and activity of a recombinant protein.

High density culture medium DNAGroTM

Catalog # Price (US$) Add to Cart   
InduXTM liquid bacterial culture Medium
500 ml/bottle, 1 bottle
40141 40
InduXTM liquid bacterial culture Medium
500 ml/bottle, 12 bottles
40142 384
InduXTM liquid bacterial culture Medium
1 liter/bottle, 1 bottle
40143 78
InduXTM liquid bacterial culture Medium
1 liter/bottle, 12 bottles
40144 748
InduXTM powdered bacterial culture Medium
powder for 1 liter/pouch, 1 pouch
40145 70
InduXTM powdered bacterial culture Medium
powder for 10 liters, 2 liters/pouch, 5 pouches
40146 560

Click on above product names for literatures of the high density bacterial E.coli culture medium InduXTM

MSDS of the high density bacterial E.coli culture medium InduXTM

Liquid culture medium MSDS
Powder culture medium MSDS

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High density bacterial E.coli culture medium SecProTM
For secretory recombinant protein production

SecProTM E.coli culture medium is proprietarily formulated for secretory protein expression. Most commonly used E.coli strains can reach OD600 = 20 in a shake flask container in this  medium. Recombinant proteins can be expressed in this medium regardless whether they are induced by IPTG, any other sugars, heat or salt. Ten times or higher cell density may be obtained in this medium when compared to that from LB broth when culture conditions are closely followed. Secretory protein expression will increase one hundred times or more. This culture medium contains trace metals, mineral, and vitamins that may be needed for solubility, stability, and activity of a recombinant protein. Some mutant cell strains or strains with toxic proteins do not grow well in this medium. In these cases, these cell strains should be grown in DetoXTM or ProGroTM medium first to reach inducible OD. Then change the medium to SecProTM for induction.

High density culture medium DNAGroTM

Catalog # Price (US$) Add to Cart   
SecProTM liquid bacterial culture Medium
500 ml/bottle, 1 bottle
40161 50
SecProTM liquid bacterial culture Medium
500 ml/bottle, 12 bottles
40162 480
SecProTM liquid bacterial culture Medium
1 liter/bottle, 1 bottle
40163 96
SecProTM liquid bacterial culture Medium
1 liter/bottle, 12 bottles
40164 920
SecProTM powdered bacterial culture Medium
powder for 1 liter/pouch, 1 pouch
40165 80
SecProTM powdered bacterial culture Medium
powder for 10 liters, 2 liters/pouch, 5 pouches
40166 640

Click on above product names for literatures of the high density bacterial E.coli culture medium SecProTM

MSDS of the high density bacterial E.coli culture medium SecProTM

Liquid culture medium MSDS
Powder culture medium MSDS

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FAQs of high density bacterial E.coli culture media

  1. Why should a high density culture medium be used for a project?
  2. How does one select a high density culture medium?
  3. Can DNAGroTM culture medium increase the yield for any plasmid?
  4. Can DNAGroTM culture medium be used for protein expression?
  5. Can chloramphenicol be used in DNAGroTM culture medium?
  6. Can ProGroTM, AutoXTM, and InduXTM culture media increase the yield for any recombinant protein?
  7. Can DetoXTM culture medium reduce the toxicity of any protein to the host cells?
  8. Why can these high density culture media increase solubility of a recombinant protein?
  9. Will these high density culture media increase solubility for any insoluble protein?
  10. Can these high density culture medium increase protein stability or activity?
  11. What are the typical conditions used for the high density culture media?
  12. What are the highest OD600 values from different shake flask containers?
  13. What are the OD600 values at normal shaking speed?
  14. Can high density culture media be used at low temperatures?
  15. What are the differences between glass and plastic flasks? How about different plastics?
  16. What are the differences between baffled and regular flasks?
  17. What are the differences among conical, round- bottom, and square-bottom tubes?
  18. Why can't I reach OD600 = 30 to 50 even though the high density media were shaken at 350 to 450 rpm?
  19. Can we use the high density media in deep-well plates? What will be the OD?
  20. Why do I sometimes get OD higher than 50?
  21. Can all high density culture media reach OD600 = 30 to 50?
  22. How do these high density culture media compare to other special media?
  23. What are the benefits of using high density culture media?

Examples of using high density culture media

bullet InduXTM increases protein yield.
bullet ProGroTM enhance protein solubility and activity.
bullet AutoXTM improve protein yield and stability.

Price list of high density E.coli culture media

High Density Growth Medium

Cat # Price (US$) Add to Cart   
DNAGroTM liquid bacterial culture Medium
500 ml/bottle, 1 bottle
40101 40
DNAGroTM liquid bacterial culture Medium
500 ml/bottle, 12 bottles
40102 384
DNAGroTM liquid bacterial culture Medium
1 liter/bottle, 1 bottle
40103 78
DNAGroTM liquid bacterial culture Medium
1 liter/bottle, 12 bottles
40104 748
DNAGroTM powdered bacterial culture Medium
powder for 1 liter/pouch, 1 pouch
40105 70
DNAGroTM powdered bacterial culture Medium
powder for 10 liters, 2 liters/pouch, 5 pouches
40106 560
ProGroTM liquid bacterial culture Medium
500 ml/bottle, 1 bottle
40111 40
ProGroTM liquid bacterial culture Medium
500 ml/bottle, 12 bottles
40112 384
ProGroTM liquid bacterial culture Medium
1 liter/bottle, 1 bottle
40113 78
ProGroTM liquid bacterial culture Medium
1 liter/bottle, 12 bottles
40114 748
ProGroTM powdered bacterial culture Medium
powder for 1 liter/pouch, 1 pouch
40115 70
ProGroTM powdered bacterial culture Medium
powder for 10 liters, 2 liters/pouch, 5 pouches
40116 560
AutoXTM liquid bacterial culture Medium
500 ml/bottle, 1 bottle
40121 40
AutoXTM liquid bacterial culture Medium
500 ml/bottle, 12 bottles
40122 384
AutoXTM liquid bacterial culture Medium
1 liter/bottle, 1 bottle
40123 78
AutoXTM liquid bacterial culture Medium
1 liter/bottle, 12 bottles
40124 748
AutoXTM powdered bacterial culture Medium
powder for 1 liter/pouch, 1 pouch
40125 70
AutoXTM powdered bacterial culture Medium
powder for 10 liters, 2 liters/pouch, 5 pouches
40126 560
DetoXTM liquid bacterial culture Medium
500 ml/bottle, 1 bottle
40131 40
DetoXTM liquid bacterial culture Medium
500 ml/bottle, 10 bottles
40132 384
DetoXTM liquid bacterial culture Medium
1 liter/bottle, 1 bottle
40133 78
DetoXTM liquid bacterial culture Medium
1 liter/bottle, 12 bottles
40134 748
DetoXTM powdered bacterial culture Medium
powder for 1 liter/pouch, 1 pouch
40135 70
DetoXTM powdered bacterial culture Medium
powder for 10 liters, 2 liters/pouch, 5 pouches
40136 560
InduXTM liquid bacterial culture Medium
500 ml/bottle, 1 bottle
40141 40
InduXTM liquid bacterial culture Medium
500 ml/bottle, 12 bottles
40142 384
InduXTM liquid bacterial culture Medium
1 liter/bottle, 1 bottle
40143 78
InduXTM liquid bacterial culture Medium
1 liter/bottle, 12 bottles
40144 748
InduXTM powdered bacterial culture Medium
powder for 1 liter/pouch, 1 pouch
40145 70
InduXTM powdered bacterial culture Medium
powder for 10 liters, 2 liters/pouch, 5 pouches
40146 560
SecProTM liquid bacterial culture Medium
500 ml/bottle, 1 bottle
40161 50
SecProTM liquid bacterial culture Medium
500 ml/bottle, 12 bottles
40162 480
SecProTM liquid bacterial culture Medium
1 liter/bottle, 1 bottle
40163 96
SecProTM liquid bacterial culture Medium
1 liter/bottle, 12 bottles
40164 920
SecProTM powdered bacterial culture Medium
powder for 1 liter/pouch, 1 pouch
40165 80
SecProTM powdered bacterial culture Medium
powder for 10 liters, 2 liters/pouch, 5 pouches
 40166 640

Warranty of high density bacterial culture  media

We guarantee our high density bacterial culture media can support E.coli culture over OD600 = 20 in a shake flask container. If the aeration conditions are carefully followed, the cell density can reach OD600 = 30 to 50. Protein and plasmid yields will increase accordingly. If our products fail to meet such specifications, Exptec will promptly replace the product. Please email us at info@exptec.com for more information.

In addition to medium contents, protein yield, solubility, stability, and activity are also related to other factors. Please click on respective words for more information.

Related products of high density culture media

Commonly used culture media
DNA ladders or DNA markers
Expression vectors
Competent cells for cloning and expression
E.coli cell strains for protein expression

Related literatures of high density culture media

Bacterial culture media
Plasmid DNA yield
Protein yield
Protein solubility
Toxic protein cloning and expression

Protocols of culture media

Plasmid DNA mini-prep
Protein induction analysis
Protein solubility analysis
Protein prep with ProGroTM medium

Notes to international customers

  1. All liquid media will be shipped to international customers only when the shipping cost is prepaid. By providing us with the account number of the preferred carrier, we can charge shipping costs directly to the customer's account. The shipping charges posted on our web site serve as packing and handling fees for international purchases.
  2. Since liquid media cannot be exposed to extreme temperatures, we will use express delivery if liquid media are ordered.
  3. Our customers may choose their preferred shipping methods. In these cases, we CANNOT guarantee the delivery conditions of the liquid media.
  4. It is our customers' responsibility to pay all the duties, taxes, and fees associated with importation and The Customs.
  5. It is important to get The Customs clearance before purchasing our products. We will not refund the purchase if the products are returned by The Customs.
  6. MSDS for each medium may be downloaded from this page under each product. MSDS may be needed for The Customs clearance.

Please email us at info@exptec.com for more information.

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