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A 80 mm X 100 mm X 0.9 mm Tris-glycine SDS gel was washed by microwave protocol (15 minutes), stained with 20 ml ProBlueTM for 10 minutes, and rinsed with H20 (2 minutes). 10 ng of BSA is clearly visible. The whole procedure is less than 30 minutes.

Larger gel will need more solution. Thicker gel will need longer staining time.

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