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tRNA
 

Different organisms use different set of genetic codons. Some heavily used codons in mammal may be rarely used in E.coli. Codon starvation results in no expression or low yield of some mammalian proteins in E.coli. tRNA Plus strains are designed to solve these problems and increase protein yield.

tRNA plus strains over-express one or more tRNA genes from E.coli. This will overcome codon starvation problems. We developed two sets of tRNA plus strains. Each set of tRNA plus strain contain one or more tRNA genes respectively. They are named PlustRNA-gene-name. tRNA-gene-name is commonly used name for tRNA genes. For example, R4 stands for tRNA gene Arg4. It is also called dnaY gene or argU tRNA gene. PlusR4 strain over expresses tRNA gene Arg4. Its tRNA contains anticodon UCU will recognize codons AGA and AGG.

Other factors also affect protein yield. Please click here for more information on protein yield.

tRNA plus strains overexpress tRNA genes:

Strain Name

Cat#

tRNA Gene

Anticodon

Rare Codon Recognition

DNA Codon

Price (US$)

PlusI2aTM 10011 Ile2(ileX) CAU AUA ATA 150
PlusL3MQ12aTM 10012 Leu3(leuW), Metm(metT), Gln1(glnU), Gln2(glnV) UAG, AUT, UUG, CUG, CUA CTA, ATG, CAA, CAG 150
PlusP2aTM 10013 Pro2(proL, proW) GGG CCC,CCU CCC, CCT 150
PlusR2S3aTM 10014 Arg2(argV), Ser3(serV) ACG, GCU CGA, AGU CGA, AGC, AGT 150
PlusR3HL1P3aTM 10015 Arg3(argT), His(hisR),
Leu(leuT), Pro3(proU)
CCG, GUG, CAG, UGG CGG, CCA, CCU CGG, CAC, CAT, CTG, CCA, CCT 150
PlusR4aTM 10016 Arg4(dnaY, argU) UCU AGA, AGG AGA, AGG 150
PlusR5aTM 10017 Arg5(argW) CCU AGG AGG 150
PlusI2bTM 10021 Ile2(ileX) CAU AUA ATA 150
PlusL3MQ12bTM 10022 Leu3(leuW), Metm(metT), Gln1(glnU), Gln2(glnV) UAG, AUT, UUG, CUG, CUA CTA, ATG, CAA, CAG 150
PlusP2bTM 10023 Pro2(proL, proW) GGG CCC,CCT CCC, CCT 150
PlusR2S3bTM 10024 Arg2(argV), Ser3(serV) ACG, GCU CGA CGA, AGC, AGT 150
PlusR3HL1P3bTM 10025 Arg3(argT), His(hisR),
Leu(leuT), Pro3(proU)
CCG, GUG, CAG, UGG CGG, CCA, CCU CGG, CAC, CAT, CTG, CCA, CCT 150
PlusR4bTM 10026 Arg4(dnaY, argU) UCU AGA, AGG AGA
AGG
150
PlusR5bTM 10027 Arg5(argW) CCU AGG AGG 150

All the strain names ending  with "a" are used in the expression with E.coli RNA polymerase and strain names ending with "b" are used in the expression with T7 RNA polymerase. All of the cell strains with TM superscripts are trademarks of Expression Technologies Inc. They are all patent-pending. These cell strains are for non-commercial research use only. Please contact us for any other uses.

Warranty

These cell strains are guaranteed to express at least three times or more than the endogenous expression level of specified gene(s). Please email us at info@exptec.com for more information.


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