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Cell Strains
 

General
tRNA
Detoxification
Heat Shock Proteins
Other Chaperones
Customized

Heterologous protein expression often encounters yield, toxicity, solubility, and stability problems. Our cell strains are specifically engineered to solve these protein expression problems. Currently all of our cell strains are E.coli strains. These E.coli strains are genetically engineered to over-express one or more genes. These over-expressed genes may increase the yield, overcome the toxicity, improve the solubility, stability, and/or activity of the recombinant proteins. Following cell strains may be used to solve one or more problems in gene cloning and protein expression.

General

These cell strains are for general cloning and expression studies.

tRNA Plus

Different organisms use different set of genetic codons. Some heavily used codons in mammal may be rarely used in E.coli. Codon starvation results in no expression or low yield of some mammalian proteins. tRNA plus strains are designed to solve these problems.

Detoxification

Many proteins have effects on cellular proliferation and differentiation. As a result, they are toxic to the host cells. Majority proteins have different degree of toxicity. The mechanism of toxicity is different for each protein. We developed detoxification cell strains to overcome toxicity of most recombinant proteins.

Heat Shock Protein

Heat shock proteins (hsps) are important in folding of many proteins. They are directly or indirectly involved in protein folding, solubility, stability, and function. We engineered cell strains over expressing human hsps.

Other Chaperones
Molecular chaperones are important in cellular protein production. Some chaperones are involved in protein folding. Others are required for protein secretion. Still others are needed for disulfide bond formation. Lack or insufficient chaperones result in non-functional, insoluble, or unstable recombinant proteins. Chaperones are critical for the function of many proteins.

Customized
We also provide customized cell strains for both academic and industrial entities. We will make E.coli strains over expressing genes you specify.


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